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rabbit anti-hbc antibody  (Agilent technologies)


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    Structured Review

    Agilent technologies rabbit anti-hbc antibody
    Rabbit Anti Hbc Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-hbc+antibody/pmc10914488-245-15-18
    Average 90 stars, based on 1 article reviews
    rabbit anti-hbc antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Incubation:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Immunofluorescence:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Transfection:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Derivative Assay:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Expressing:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Mutagenesis:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Plasmid Preparation:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Negative Control:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Southern Blot:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Labeling:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Western Blot:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).

    Immunoprecipitation:

    Article Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
    Article Snippet: For immunofluorescence analysis (IFA) at day 6 post-infection, cells were fixed overnight with 4% paraformaldehyde and permeabilized with 0.075% Triton X-100 in PBS and then blocked with 5% bovine serum albumin (BSA) in PBS at 4°C for 16 h. Cells were incubated with rabbit anti-HBc antibody (DAKO, USA) for 2 h, repeatedly washed and subsequently incubated with secondary antibody, anti-rabbit-IgG conjugated with Alexa Fluor 488.

    Article Title: CpAMs induce assembly of HBV capsids with altered electrophoresis mobility: Implications for mechanism of inhibiting pgRNA packaging
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (Cat. No. B0586).

    Article Title: Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly
    Article Snippet: Rabbit anti-HBc antibody was obtained from Dako (catalog number B0586).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg/capsid protein was detected 299 using rabbit anti-HBc antibody (Dako, 1:1000 dilution) as described for HBcAg/p21 (53-300 55).


    Article Title: Oxygen-dependent histone lysine demethylase 4 restricts hepatitis B virus replication
    Article Snippet: Cells were permeabilized (0.1% Triton-X100, 30 min), blocked (3% BSA, 30 min), then stained with rabbit anti-HBc antibody (Dako) for 1 h and a 488-conjugated anti-rabbit secondary antibody (Thermofisher) for 40 min. Coverslips were incubated in DAPI for 10 min before washing and mounting using Prolong Anti-fade (LifeTechnologies).

    Article Title: PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation
    Article Snippet: HBcAg was immunoprecipitated with 1 μl rabbit anti-HBc antibody (from Adam Zlotnick, Indiana University) plus protein G-agarose (Santa Cruz Biotechnology) according to the manufacturer's instructions (Abcam), resolved by SDS-PAGE, transferred onto polyvinylidene difluoride (PVDF) membranes, and identified by using rabbit anti-HBc antibody (1:1,000 dilution; Dako), followed by incubation with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Cell Signaling Technology).



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    Enhancing expression of Id1 inhibited HBV transcription and replication in vitro and in vivo . (A) Overexpressing Id1 inhibited HBV DNA intermediates extracted from intracellular nucleocapsid 3 days after HepG2.2.15 cells infected with Id1 adeno-associated virus (Id1Ad). HBV DNA subsequently were subjected to qPCR and Southern blotting. The lysates from cells infected with Id1Ad were analyzed by western blotting with anti-Id1 and <t>anti-HBc</t> antibodies. GAPDH expression was used as loading control. HBeAg ELISA were used to screen culture supernatants 3 days after transfection. ** P < 0.01. n = 5. (B) HBV covalently closed circle DNA (cccDNA) and pgRNA were subjected to PCR quantification using specific primers with CA2 and GAPDH as the reference gene, respectively. * P < 0.05 and ** P < 0.01. n = 5. (C) HepG2-NTCP cells were firstly infected with 800 HBV viral particles/cell for 24 hours, and followed by transfection with pcDNA3.1-Id1 plasmid or pcDNA3.1 vector or medication with ETV. HBV DNA in cytoplasm was measured by qPCR after culture for additional 96 hours. All the qPCR data are presented as the mean±SE of triplicate experiments. ** P < 0.01. n = 5. (D) Effect of Id1 overexpression on four HBV promoters. Different luciferase reporter vectors were co-transfected with Id1Ad or GFPAd into HepG2 cells. pRL-TK was co-transfected to normalise the transfection efficiency. The luciferase activity was measured at 48 h post-transfection. * P < 0.05. n = 5. (E) The mice were randomly allotted to two groups of 5 individuals per group. 7×10 9 GFU Id1Ad (n1-n5) or GFPAd (n6-n10) were dissolved in 0.3 ml 0.9% normal saline and injected through the tail vein into mice. The mice were sacrificed at 20 days after injection, and the liver tissue and serum were collected. Intracellular HBV DNA extracted from 10 mg liver tissue was analyzed by southern blotting (top panel). The expression of Id1 and HBc in liver tissue lysates was tested by Western blotting with β-actin as an internal control (bottom panel). (F) The relative level of HBeAg in serum samples of AAV/HBV-infected mice were subjected to ELISA kits, ** P <0.01. n = 5.
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    Image Search Results


    Detection of HBcAg and HiBiT-tagged viral proteins by transfection of HiBiT-tagged HBVcc plasmids. ( A ) The production of HBcAg and HiBiT-tagged viral proteins was detected by immunostaining with anti-HBc and anti-HiBiT antibodies 3 days after transfection. Nuclei were visualized by staining with DAPI. The white bar indicates 100 µM. ( B ) The HiBiT signals in cells and culture medium were measured after transfection of plasmids for HiBiT-tagged HBVcc. HBVcc-WT was used as a negative control. ( C ) HBsAg production in the culture medium was measured after transfection of plasmids for HiBiT-tagged HBVcc.

    Journal: mSphere

    Article Title: Exploring the tolerable region for HiBiT tag insertion in the hepatitis B virus genome

    doi: 10.1128/msphere.00518-24

    Figure Lengend Snippet: Detection of HBcAg and HiBiT-tagged viral proteins by transfection of HiBiT-tagged HBVcc plasmids. ( A ) The production of HBcAg and HiBiT-tagged viral proteins was detected by immunostaining with anti-HBc and anti-HiBiT antibodies 3 days after transfection. Nuclei were visualized by staining with DAPI. The white bar indicates 100 µM. ( B ) The HiBiT signals in cells and culture medium were measured after transfection of plasmids for HiBiT-tagged HBVcc. HBVcc-WT was used as a negative control. ( C ) HBsAg production in the culture medium was measured after transfection of plasmids for HiBiT-tagged HBVcc.

    Article Snippet: The expression of HBcAg in transfected cells was detected by staining with a rabbit polyclonal anti-HBc antibody (Beacle Inc., Kyoto, Japan) and Alexa Fluor 555-conjugated anti-rabbit IgG (Thermo Fisher Scientific).

    Techniques: Transfection, Immunostaining, Staining, Negative Control

    Infection of HiBiT-tagged HBVcc in human primary hepatocytes. ( A ) Human primary hepatocytes were infected with HiBiT-tagged HBVcc at 200 GEq/cell, and the infection efficiencies were compared with those of HBVcc-WT by monitoring HBsAg and HiBiT signals in culture medium on the indicated days after infection. ( B ) The HBV DNA levels in the culture media of HBVcc-WT- and HiBiT-tagged HBVcc-infected cells were assessed by real-time PCR with a primer and probe set designed to target the HBs region after treatment with DNase after 12 days of culture. ( C ) HBVcc-WT- and HiBiT-tagged HBVcc-infected cells were detected by staining with rabbit polyclonal anti-HBc antibody and Alexa Fluor 555-conjugated anti-rabbit IgG. Nuclei were visualized by staining with DAPI.

    Journal: mSphere

    Article Title: Exploring the tolerable region for HiBiT tag insertion in the hepatitis B virus genome

    doi: 10.1128/msphere.00518-24

    Figure Lengend Snippet: Infection of HiBiT-tagged HBVcc in human primary hepatocytes. ( A ) Human primary hepatocytes were infected with HiBiT-tagged HBVcc at 200 GEq/cell, and the infection efficiencies were compared with those of HBVcc-WT by monitoring HBsAg and HiBiT signals in culture medium on the indicated days after infection. ( B ) The HBV DNA levels in the culture media of HBVcc-WT- and HiBiT-tagged HBVcc-infected cells were assessed by real-time PCR with a primer and probe set designed to target the HBs region after treatment with DNase after 12 days of culture. ( C ) HBVcc-WT- and HiBiT-tagged HBVcc-infected cells were detected by staining with rabbit polyclonal anti-HBc antibody and Alexa Fluor 555-conjugated anti-rabbit IgG. Nuclei were visualized by staining with DAPI.

    Article Snippet: The expression of HBcAg in transfected cells was detected by staining with a rabbit polyclonal anti-HBc antibody (Beacle Inc., Kyoto, Japan) and Alexa Fluor 555-conjugated anti-rabbit IgG (Thermo Fisher Scientific).

    Techniques: Infection, Real-time Polymerase Chain Reaction, Staining

    Infection of HepG2/NTCP cells with HiBiT-tagged HBVcc.( A ) HepG2/NTCP cells were infected with HiBiT-tagged HBVcc at 200 GEq/cell, and the infection efficiencies were compared with those of HBVcc-WT by monitoring HBsAg and HiBiT signals in the culture media on the indicated days after infection. ( B ) The HBV DNA levels in the culture media of HBVcc-WT- and HiBiT-tagged HBVcc-infected cells were assessed by real-time PCR with a primer and probe set designed to target the HBs region after treatment with DNase after 12 days of culture. ( C ) HBVcc-WT- and HiBiT-tagged HBVcc-infected cells were detected by staining with rabbit polyclonal anti-HBc antibody and Alexa Fluor 555-conjugated anti-rabbit IgG. Nuclei were visualized by staining with DAPI.

    Journal: mSphere

    Article Title: Exploring the tolerable region for HiBiT tag insertion in the hepatitis B virus genome

    doi: 10.1128/msphere.00518-24

    Figure Lengend Snippet: Infection of HepG2/NTCP cells with HiBiT-tagged HBVcc.( A ) HepG2/NTCP cells were infected with HiBiT-tagged HBVcc at 200 GEq/cell, and the infection efficiencies were compared with those of HBVcc-WT by monitoring HBsAg and HiBiT signals in the culture media on the indicated days after infection. ( B ) The HBV DNA levels in the culture media of HBVcc-WT- and HiBiT-tagged HBVcc-infected cells were assessed by real-time PCR with a primer and probe set designed to target the HBs region after treatment with DNase after 12 days of culture. ( C ) HBVcc-WT- and HiBiT-tagged HBVcc-infected cells were detected by staining with rabbit polyclonal anti-HBc antibody and Alexa Fluor 555-conjugated anti-rabbit IgG. Nuclei were visualized by staining with DAPI.

    Article Snippet: The expression of HBcAg in transfected cells was detected by staining with a rabbit polyclonal anti-HBc antibody (Beacle Inc., Kyoto, Japan) and Alexa Fluor 555-conjugated anti-rabbit IgG (Thermo Fisher Scientific).

    Techniques: Infection, Real-time Polymerase Chain Reaction, Staining

    Enhancing expression of Id1 inhibited HBV transcription and replication in vitro and in vivo . (A) Overexpressing Id1 inhibited HBV DNA intermediates extracted from intracellular nucleocapsid 3 days after HepG2.2.15 cells infected with Id1 adeno-associated virus (Id1Ad). HBV DNA subsequently were subjected to qPCR and Southern blotting. The lysates from cells infected with Id1Ad were analyzed by western blotting with anti-Id1 and anti-HBc antibodies. GAPDH expression was used as loading control. HBeAg ELISA were used to screen culture supernatants 3 days after transfection. ** P < 0.01. n = 5. (B) HBV covalently closed circle DNA (cccDNA) and pgRNA were subjected to PCR quantification using specific primers with CA2 and GAPDH as the reference gene, respectively. * P < 0.05 and ** P < 0.01. n = 5. (C) HepG2-NTCP cells were firstly infected with 800 HBV viral particles/cell for 24 hours, and followed by transfection with pcDNA3.1-Id1 plasmid or pcDNA3.1 vector or medication with ETV. HBV DNA in cytoplasm was measured by qPCR after culture for additional 96 hours. All the qPCR data are presented as the mean±SE of triplicate experiments. ** P < 0.01. n = 5. (D) Effect of Id1 overexpression on four HBV promoters. Different luciferase reporter vectors were co-transfected with Id1Ad or GFPAd into HepG2 cells. pRL-TK was co-transfected to normalise the transfection efficiency. The luciferase activity was measured at 48 h post-transfection. * P < 0.05. n = 5. (E) The mice were randomly allotted to two groups of 5 individuals per group. 7×10 9 GFU Id1Ad (n1-n5) or GFPAd (n6-n10) were dissolved in 0.3 ml 0.9% normal saline and injected through the tail vein into mice. The mice were sacrificed at 20 days after injection, and the liver tissue and serum were collected. Intracellular HBV DNA extracted from 10 mg liver tissue was analyzed by southern blotting (top panel). The expression of Id1 and HBc in liver tissue lysates was tested by Western blotting with β-actin as an internal control (bottom panel). (F) The relative level of HBeAg in serum samples of AAV/HBV-infected mice were subjected to ELISA kits, ** P <0.01. n = 5.

    Journal: International Journal of Biological Sciences

    Article Title: Cellular Id1 inhibits hepatitis B virus transcription by interacting with the novel covalently closed circular DNA-binding protein E2F4

    doi: 10.7150/ijbs.62106

    Figure Lengend Snippet: Enhancing expression of Id1 inhibited HBV transcription and replication in vitro and in vivo . (A) Overexpressing Id1 inhibited HBV DNA intermediates extracted from intracellular nucleocapsid 3 days after HepG2.2.15 cells infected with Id1 adeno-associated virus (Id1Ad). HBV DNA subsequently were subjected to qPCR and Southern blotting. The lysates from cells infected with Id1Ad were analyzed by western blotting with anti-Id1 and anti-HBc antibodies. GAPDH expression was used as loading control. HBeAg ELISA were used to screen culture supernatants 3 days after transfection. ** P < 0.01. n = 5. (B) HBV covalently closed circle DNA (cccDNA) and pgRNA were subjected to PCR quantification using specific primers with CA2 and GAPDH as the reference gene, respectively. * P < 0.05 and ** P < 0.01. n = 5. (C) HepG2-NTCP cells were firstly infected with 800 HBV viral particles/cell for 24 hours, and followed by transfection with pcDNA3.1-Id1 plasmid or pcDNA3.1 vector or medication with ETV. HBV DNA in cytoplasm was measured by qPCR after culture for additional 96 hours. All the qPCR data are presented as the mean±SE of triplicate experiments. ** P < 0.01. n = 5. (D) Effect of Id1 overexpression on four HBV promoters. Different luciferase reporter vectors were co-transfected with Id1Ad or GFPAd into HepG2 cells. pRL-TK was co-transfected to normalise the transfection efficiency. The luciferase activity was measured at 48 h post-transfection. * P < 0.05. n = 5. (E) The mice were randomly allotted to two groups of 5 individuals per group. 7×10 9 GFU Id1Ad (n1-n5) or GFPAd (n6-n10) were dissolved in 0.3 ml 0.9% normal saline and injected through the tail vein into mice. The mice were sacrificed at 20 days after injection, and the liver tissue and serum were collected. Intracellular HBV DNA extracted from 10 mg liver tissue was analyzed by southern blotting (top panel). The expression of Id1 and HBc in liver tissue lysates was tested by Western blotting with β-actin as an internal control (bottom panel). (F) The relative level of HBeAg in serum samples of AAV/HBV-infected mice were subjected to ELISA kits, ** P <0.01. n = 5.

    Article Snippet: Rabbit anti-HBc poly-clonal antibody (B0586) was obtained from Dako (Denmark).

    Techniques: Expressing, In Vitro, In Vivo, Infection, Southern Blot, Western Blot, Enzyme-linked Immunosorbent Assay, Transfection, Plasmid Preparation, Over Expression, Luciferase, Activity Assay, Injection